| 產(chǎn)品編號 | bsm-61187R |
| 英文名稱 | TSPO Recombinant Rabbit mAb |
| 中文名稱 | TSPO蛋白重組兔單抗 |
| 別 名 | TSPO; PBR; BZRP; IBP; MBR; PKBS; Mitochondrial benzodiazepine receptor; Peripheral benzodiazepine receptor. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 7C7 |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 19 kDa |
| 檢測分子量 | 18 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human TSPO: 100-169 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Can bind protoporphyrin IX and may play a role in the transport of porphyrins and heme.Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism, but its precise physiological role is controversial. It is apparently not required for steroid hormone biosynthesis. Was initially identified as peripheral-type benzodiazepine receptor; can also bind isoquinoline carboxamides. SWISS: P30536 Gene ID: 706 地西泮結(jié)合抑制因子； DBI能拮抗安定物質(zhì)：diazepam與腦突觸膜相結(jié)合 ,故稱之為安定結(jié)合抑制蛋白(DBI)，地西泮結(jié)合抑制因子(diazepam binding inhibitor,DBI)具有抑制由葡萄糖誘導(dǎo)的胰島素分泌、促進膽固醇跨線粒體膜轉(zhuǎn)運和調(diào)節(jié)脂肪酸合成與代謝等多種生理功能。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with TSPO monoclonal antibody, unconjugated (bsm-61187R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (TSPO) monoclonal Antibody, unconjugated (bsm-61187R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
