| 產(chǎn)品編號 | bs-0683R |
| 英文名稱 | LRRK2 Rabbit pAb |
| 中文名稱 | 富亮氨酸重復(fù)激酶2抗體 |
| 別 名 | Dardarin; Leucine rich repeat kinase 2; LRRK 2; LRRK-2; PARK 8; PARK8; ROCO 2; ROCO-2; ROCO2; Leucine-rich repeat serine/threonine-protein kinase 2; Dardarin; LRRK2_HUMAN; augmented in rheumatoid arthritis 17; AURA17; Leucine rich repeat kinase 2; RIPK7. |
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Specific References (1) | bs-0683R has been referenced in 1 publications.
[IF=2.94] Zhang, Shuai-nan, et al. "Cerebral potential biomarkers discovery and metabolic pathways analysis of α-synucleinopathies and the dual effects of Acanthopanax senticosus Harms on central nervous system through metabolomics analysis." Journal of ethnopharmacology (2015). WB ; Mouse.
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| 研究領(lǐng)域 | 神經(jīng)生物學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Cow) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 286 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LRRK2: 2455-2527/2527 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
LRRK2 (Dardarin) is encoded by the gene LRRK2. Genetic mutations of LRRK2 have been linked to Parkinsonism and synucleinopathies. LRRK2 belongs to the ROCO protein family and includes a protein kinase domain of the MAPKKK class and several other major functional domains. Function: May play a role in the phosphorylation of proteins central to Parkinson disease. Phosphorylates PRDX3. May also have GTPase activity. Subcellular Location: Cytoplasm. Membrane. Mitochondrion. Localized in the cytoplasm and associated with cellular membrane structures. Associates with the mitochondrial outer membrane. Tissue Specificity: Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas. DISEASE: Defects in LRRK2 are the cause of Parkinson disease type 8 (PARK8) [MIM:607060]. A slowly progressive neurodegenerative disorder characterized by bradykinesia, rigidity, resting tremor, postural instability, neuronal loss in the substantia nigra, and the presence of neurofibrillary MAPT (tau)-positive and Lewy bodies in some patients. Similarity: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 12 LRR (leucine-rich) repeats. Contains 1 protein kinase domain. Contains 1 Roc domain. SWISS: Q5S007 Gene ID: 120892 Database links: Entrez Gene: 120892 Human Entrez Gene: 66725 Mouse Omim: 609007 Human SwissProt: Q5S007 Human SwissProt: Q5S006 Mouse Unigene: 187636 Human Unigene: 37558 Mouse Unigene: 213971 Rat 主要用于：帕金森氏病的研究 亮氨酸豐富區(qū)重復(fù)激酶2 |
| 產(chǎn)品圖片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-LRRK2 Polyclonal Antibody, Unconjugated(bs-0683R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: A549(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
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