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產(chǎn)品編號(hào)	bs-0287R
英文名稱	Rabbit Anti-His tag antibody
中文名稱	His tag標(biāo)簽抗體
別名	HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.
	Specific References (17) \| bs-0287R has been referenced in 17 publications. [IF=2.914] WB ; Pig. PubMed:32649964 [IF=19.924] Yifan Yang. et al. A Versatile Platform for the Tumor-Targeted Intracellular Delivery of Peptides, Proteins, and siRNA. ADV FUNCT MATER. 2023 Apr;:2301011 WB ; Human. PubMed:10.1002/adfm.202301011 [IF=7.2] Zhiyan Hu. et al. CIP4 targeted to recruit GTP-Cdc42 involving in invadopodia formation via NF-κB signaling pathway promotes invasion and metastasis of CRC. Mol Ther-Oncolytics. 2022 Feb;: WB ; Strain（Bl21）. PubMed:10.1016/j.omto.2022.02.023 [IF=4.38] Ma, Xiangyi, et al. "DT390-triTMTP1, a novel fusion protein of diphtheria toxin with tandem repeat TMTP1 peptide, preferentially targets metastatic tumors." Molecular pharmaceutics 10.1 (2013): 115-126. IHC-P ; PubMed:23198829 [IF=4.235] Xu Zhenshang. et al. Comparison of Enzyme Secretion and Ferulic Acid Production by Escherichia coli Expressing Different Lactobacillus Feruloyl Esterases. Front Microbiol. 2020 Nov;11:2281 WB ; PubMed:33329424 [IF=3.309] Chuandong Cheng. et al. SREBP2/Rab11s/GLUT1/6 network regulates proliferation and migration of glioblastoma. PATHOL RES PRACT. 2022 Oct;:154176 WB ; Human. PubMed:36327817 [IF=2.984] Ruirui Luoet al. Clostridium perfringens beta2 toxin induced in vitro oxidative damage and its toxic assessment in porcine small intestinal epithelial cell lines. Gene . 2020 Oct 30;759:144999. WB ; pig. PubMed:32717305 [IF=2.984] WB, IF ; Pig. PubMed:32717305 [IF=2.914] Xiaoli Gao. et al. Effects of Clostridium perfringens beta2 toxin on apoptosis, inflammation, and barrier function of intestinal porcine epithelial cells. Microb Pathogenesis. 2020 Oct;147:104379 WB ; Pig. PubMed:32649964 [IF=2.913] Li N et al. Identification and characterization of an atypical RIG-I encoded by planarian Dugesia japonica and its essential role in the immune response.(2019) Dev Comp Immunol. Feb;91:72-84. WB ; PubMed:30355517 [IF=2.7] Peng, Wang, et al. "Attenuation of specific CTL responses by highly efficient transduction of the recombinant adenovirus expressing His-tag-ICP47 fusion gene." Life Science Journal 10.1 (2013). WB ; PubMed:Attenuation of specific CTL responses by highly efficient transduction of the recombinant adenovirus expressing His-tag-ICP47 fusion gen [IF=2.7] WANG, Peng, et al. "Construction and identification of a recombinant adenovirus vector expressing His-tag-ICP47 fusion gene." Life Science Journal 9.1 (2012). PubMed:Life Science Journal 9.1 (2012) [IF=2.56] Wang, P. et al. "Attenuation of cytokine production of interleukine -2 and perforin in Two-way mixed lymphocyte reaction by highly efficient transduction of the recombinant adenovirus expressing ICP47 gene."Life Sci J 2013;10(3):963-969 WB ; PubMed:ISSN:1097-8135 [IF=1.74] Wang, Peng, et al. "Recombinant adenovirus expressing ICP47 gene suppresses the ability of dendritic cells by restricting specific T cell responses." Cellular Immunology (2013). WB ; PubMed:23774531 [IF=1.676] Ma et al. Adjuvant effects mediated by the carbohydrate recognition domain of Agrocybe aegerita lectin interacting with avian influenza H9N2 viral surface glycosylated proteins. (2017) J.Zhejiang.Univ.Sci.. 18:653-661 other ; PubMed:28786240 [IF=1.109] He,et al.Recombinant Fiber-2 Protein-Based Indirect ELISA for Antibody Detection of Fowl Adenovirus Serotype 4.(2018) Avian Diseases. 62:73-78. WB ; PubMed:29620471 [IF=0] Ren et al. Long-term rescue of rat retinal ganglion cells and visual function by AAV-mediated BDNF expression after acute elevation of intraocular pressure. (2012) Invest.Ophthalmol.Vis.Sci. 53:1003-11 IHC ; PubMed:22247466
產(chǎn)品類型	標(biāo)簽抗體
研究領(lǐng)域	腫瘤細(xì)胞生物免疫學(xué) 染色質(zhì)和核信號(hào) 信號(hào)轉(zhuǎn)導(dǎo) 合成與降解結(jié)合蛋白
抗體來(lái)源	Rabbit
克隆類型	Polyclonal
交叉反應(yīng)	Recombinant protein
產(chǎn)品應(yīng)用	WB=1:500-2000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
性狀	Liquid
濃度	1mg/ml
免疫原	Synthetic peptide: HHHHHH conjugated to KLH
亞型	IgG
純化方法	affinity purified by Protein A
緩沖液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng)	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產(chǎn)品介紹	The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. SWISS: N/A Gene ID: N/A 標(biāo)簽抗體蛋白質(zhì)純化的新途徑─應(yīng)用His-Tag抗體檢測(cè)His-Tag融合蛋白 1） 6xHis與不同的Sepharose純化填料結(jié)合（捆綁），可不受構(gòu)象的影響，在天然或變形的條件下可進(jìn)行進(jìn)一步純化。 2）可在溫和的洗脫液環(huán)境中，其結(jié)合、洗滌及洗脫步驟不會(huì)對(duì)蛋白結(jié)構(gòu)產(chǎn)生影響。 3） 6xHis標(biāo)簽比普通（Tag）要小得多，經(jīng)純化后的蛋白可直接進(jìn)行下游操作?？捎糜谌魏伪磉_(dá)系統(tǒng)（包括：細(xì)菌，桿狀病毒和哺乳動(dòng)物） 4） 6xHis標(biāo)簽在生理溶液pH下不帶電荷，其標(biāo)簽不會(huì)干涉重組蛋白的結(jié)構(gòu)和功能， 6xHis不會(huì)影響蛋白分泌 5） 6xHis標(biāo)簽免疫原性差，重組蛋白可以不必除去標(biāo)簽而直接作為抗原進(jìn)行免疫
產(chǎn)品圖片	20 ng rGFP-His protein (bs-33009P) per lane probed with His tag polyclonal antibody respectively, unconjugated (bs-0287R) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.

	免疫組化常用試劑
	免疫印跡常用試劑
	各種標(biāo)記二抗

	1、抗體溶解方法
	2、抗體修復(fù)方式
	3、常用試劑的配制
	4、免疫組化操作步驟
	5、免疫組化問(wèn)題解答
	6、Western Blotting 操作步驟
	7、Western Blotting 問(wèn)題解答
	8、關(guān)于肽鏈的設(shè)計(jì)
	9、多肽的溶解與保存
	10、酶標(biāo)抗體效價(jià)測(cè)定程序

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