| 產品編號 | bs-0816R |
| 英文名稱 | Lysozyme Rabbit pAb |
| 中文名稱 | 溶菌酶抗體 |
| 別 名 | 1 4 beta n acetylmuramidase c; 14 beta N acetylmuramidase; 14 beta N acetylmuramidase C; EC 3.2.1.17; lysosyme; Lysozyme C; Lysozyme C precursor; Lyz; LZM; Muramidase. |
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Specific References (3) | bs-0816R has been referenced in 3 publications.
[IF=8.713] Peng Yang. et al. Single-cell RNA sequencing analysis of shrimp immune cells identifies macrophage-like phagocytes. ELIFE. 2022 Oct WB ; Shrimp.
[IF=3.757] Haruna Nakayama. et al. Preparing a pore-size-controlled TEMPO oxidized cellulose nanofiber substrate for enzyme immunoassay applications. PROCESS BIOCHEM. Process Biochem. 2022 Jun;117:174 ELISA ; Material.
[IF=1.967] Nagata, Kenichiro. et al. Analysis of the enzymatic degradation of lysozyme fibrils using a combination method of non-denaturing gel electrophoresis and double staining with Coomassie Brilliant Blue G-250 and R-250 dyes. ANAL SCI. 2022 Nov;:1-8 WB ;
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| 研究領域 | 腫瘤 細胞生物 免疫學 激酶和磷酸酶 合成與降解 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse,Rat (predicted: Chicken) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 17 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Full length native Lysozyme: purufied protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Lysozyme catalyzes the hydrolysis of certain mucopolysaccharides of bacterial cell walls. Specifically, it catalyzes the hydrolysis of the bacterial cell wall beta glycosidic linkages between N acetylmuramic acid and N acetylglucosamine. It is found in spleen, lung, kidney, white blood cells, plasma, saliva, milk, and tears. Subcellular Location: Secreted. DISEASE: Defects in LYZ are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. Similarity: Belongs to the glycosyl hydrolase 22 family. Database links: Entrez Gene: 4069 Human Entrez Gene: 17105 Mouse Entrez Gene: 17110 Mouse Omim: 153450 Human SwissProt: P61626 Human SwissProt: P08905 Mouse Unigene: 524579 Human 溶菌酶（lysozyme ）又稱細胞壁質酶（muramidase）或n-乙酰胞壁質聚糖水解酶（n-acetylmuramideglycanohydralase）。 對于哺乳動物溶菌酶，其牛、豬、貓、兔、猴、馬、羊等動物的乳汁中分離出溶菌酶，其化學性質與人溶菌酶相似。 溶菌酶作為機體非特異免疫因子之一，參與機體多種免疫反應，在機體正常防御功能和非特異免疫中，具有保持機體生理平衡的重要作用?？筛纳坪驮鰪娋奘燃毎淌珊拖δ?，激活白細胞吞噬功能，并能改善細胞抑制劑所導致的白細胞減少，從而增強機體的抵抗力。 |
| 產品圖片 |
Sample:
Raw264.7 Cell (Mouse) Lysate at 40 ug
HL-60 Cell (Human)Lysate at 40 ug
Primary: Anti-Lysozyme (bs-0816R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 16 kD
Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Lysozyme Polyclonal Antibody, Unconjugated(bs-0816R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Lysozyme Polyclonal Antibody, Unconjugated(bs-0816R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
