| 產(chǎn)品編號 | bs-1159R |
| 英文名稱 | P-cadherin Rabbit pAb |
| 中文名稱 | P-鈣粘附分子抗體 |
| 別 名 | placental; Cadherin-3; P cadherin; Cadp; Placental cadherin; P-cadherin; AI385538; Pca; Cadherin 3 precursor; Cadherin 3 type 1; Calcium dependent adhesion protein placental; Cadherin; CDH3; CDHP; 7B4 antigen; Cadherin-1; Cadherin-2; Cadherin-4; Cadherin-5; CAM 120/80; CD144; CD324; CD325; Cdh4; CDH5; CDHE; CDHN; CADH3_HUMAN; E-cadherin; Epithelial cadherin; N-cadherin; NCAD; Neural cadherin; P-cadherin; UVO; Uvomorulin; Vascular endothelial cadherin; CDH 3; CDH3; CDH3 protein; CDHP; HJMD; PCAD; Placental cadherin. |
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Specific References (1) | bs-1159R has been referenced in 1 publications.
[IF=5.232] Chen F et al. Integrated Analysis of Quantitative Proteome and Transcriptional Profiles Reveals the Dynamic Function of Maternally Expressed Proteins After Parthenogenetic Activation of Buffalo Oocyte.Mol Cell Proteomics. 2018 Oct;17(10):1875-1891. WB ; Buffalo.
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| 研究領域 | 細胞生物 免疫學 細胞粘附分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Cow,Chicken,Dog,GuineaPig) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 80 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human P-cadherin: 625-725/829 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
cell-cell adhesion molecules. Cadherins are responsible for a whole range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation. N-cadherin is found in many locations including cardiac adherins junctions, oral squamous epithelial cells, and breast epithelial cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin. Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Function: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Subunit: Interacts with CDCP1. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed in some normal epithelial tissues and in some carcinoma cell lines. DISEASE: Defects in CDH3 are the cause of hypotrichosis with juvenile macular dystrophy (HJMD) [MIM:601553]. HJMD is a rare autosomal recessive disorder characterized by early hair loss heralding severe degenerative changes of the retinal macula and culminating in blindness during the second to third decade of life. Defects in CDH3 are the cause of ectodermal dysplasia with ectrodactyly and macular dystrophy (EEM) [MIM:225280]; also known as EEM syndrome, Albrectsen-Svendsen syndrome or Ohdo-Hirayama-Terawaki syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EEM is an autosomal recessive condition characterized by features of ectodermal dysplasia such as sparse eyebrows and scalp hair, and selective tooth agenesis associated with macular dystrophy and ectrodactyly. Similarity: Contains 5 cadherin domains. SWISS: P22223 Gene ID: 1001 Database links: Entrez Gene: 1001 Human Omim: 114021 Human SwissProt: P22223 Human Unigene: 191842 Human |
| 產(chǎn)品圖片 |
Sample:
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti-P-cadherin (bs-1159R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 90 kD
Tissue/cell: U251 cells;4% Paraformaldehyde-fixed;
Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-P-cadherin Polyclonal Antibody, Unconjugated(bs-1159R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Image provided by Independent Validation (badge 029741). Histogram of human DU145 and human LNCaP cells stained with Rabbit Anti-P-cadherin Polyclonal Antibody (orange)(bs-1159R at 1:100), isotype control antibody (green), secondary antibody only (blue) and unstained (red)
Overlay histogram showing Mouse SP2/0 stained with bs-1159R-PE (red line). The cells were fixed with 1% paraformaldehyde (10 min). The cells were then incubated with the antibody(bs-1159R-PE, 2ug/1x106cells) for 30 min at 22-25°C. Isotype control antibody (black line) was rabbit IgG (2ug/1x106cells) used under the same conditions. Acquisition of >5,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
