| 產(chǎn)品編號 | bs-1098R |
| 英文名稱 | Urokinase Rabbit pAb |
| 中文名稱 | 尿激酶型纖溶酶原激活因子抗體 |
| 別 名 | Urokinase; ATF; ATF uPA; PLAU; UPA; Plasminogen activator; Plasminogen activator urinary; Plasminogen activator urokinase; u PA; U plasminogen activator; UPA; URK; Urokinase plasminogen activator; Urokinase type plasminogen activator; Urokinase type plasminogen activator precursor. |
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Specific References (1) | bs-1098R has been referenced in 1 publications.
[IF=5.123] Liu XP et al. A Label-free Photoelectrochemical Biosensor for Urokinase-type Plasminogen Activator Detection Based on a gC 3 N 4/CdS Nanocomposite.Analytica Chimica Acta.1025;99-107. Photoelectrochemical detection ;
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| 研究領(lǐng)域 | 細胞生物 免疫學 神經(jīng)生物學 激酶和磷酸酶 通道蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 49 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PLAU: 361-432/432 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Urokinase is a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in the Urokinase gene may be associated with late onset Alzheimer disease and also with decreased affinity for fibrin binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg Val bond in plasminogen. The proprotein is cleaved at a Lys Ile bond by plasmin to form a two chain derivative in which a single disulfide bond connects the amino terminal A chain to the catalytically active, carboxy terminal B chain. This two chain derivative is also called HMW uPA (high molecular weight uPA). HMW uPA can be further processed into LMW uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino terminal fragment. LMW uPA is proteolytically active but does not bind to the uPA receptor. Function: Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. Subcellular Location: Secreted. Tissue Specificity: Expressed in the prostate gland and prostate cancers. Post-translational modifications: Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. Similarity: Belongs to the peptidase S1 family. Contains 1 EGF-like domain. Contains 1 kringle domain. Contains 1 peptidase S1 domain. SWISS: P00749 Gene ID: 5328 Database links: Entrez Gene: 5328 Human Entrez Gene: 18792 Mouse Omim: 191840 Human SwissProt: P00749 Human SwissProt: P06869 Mouse Unigene: 77274 Human Unigene: 4183 Mouse Unigene: 6064 Rat 尿激酶型纖溶酶激活因子;是一種多效細胞因子，參與多種病理生理過程；uPA系統(tǒng)可通過調(diào)節(jié)纖溶酶的活性降解p淀粉樣蛋白;是一種絲氨酸蛋白酶，將血漿酶原轉(zhuǎn)化成血漿酶（纖維蛋白溶酶）。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Placenta (Mouse) Lysate at 40 ug
Lane 2: Liver (Mouse) Lysate at 40 ug
Lane 3: Lung (Mouse) Lysate at 40 ug
Lane 4: Spleen (Mouse) Lysate at 40 ug
Lane 5: Liver (Rat) Lysate at 40 ug
Lane 6: Lung (Rat) Lysate at 40 ug
Lane 7: Spleen (Rat) Lysate at 40 ug
Primary: Anti-Urokinase (bs-1098R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40-80 kD
Observed band size: 45 kD
Sample:
Cerebrum (mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-Urokinase (bs-1098R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49kD
Observed band size: 48kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-Urokinase (bs-1098R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PLAU/UPA/Urokinase Polyclonal Antibody, Unconjugated(bs-1098R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
