| 產品編號 | bs-1508R |
| 英文名稱 | Cathepsin L Rabbit pAb |
| 中文名稱 | 組織蛋白酶L抗體 |
| 別 名 | Cathepsin L heavy chain; Cathepsin L light chain; CathepsinL; CATL; CTSL; CTSL protein; DKFZp686A18159; FLJ31037; Major excreted protein; MEP; cath-L; CL; CATL1_HUMAN; CathepsinL1; Cathepsin L1, Cathepsin-L1; CTSL 1; CTSL-1, CTS L1, CTS-L1. |
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Specific References (2) | bs-1508R has been referenced in 2 publications.
[IF=7.56] Hernáez B, Guerra M, Salas ML, Andrés G (2016) African Swine Fever Virus Undergoes Outer Envelope Disruption, Capsid Disassembly and Inner Envelope Fusion before Core Release from Multivesicular Endosomes. PLoS Pathog 12(4): e1005595. other ; Pig.
[IF=4.406] Mathew Suji Eapen. et al. The Pathophysiology of COVID-19 and SARS-CoV-2 Infection: Dysregulation of endocytic machinery and ACE2 in small airways of smokers and COPD patients can augment their susceptibility to SARS-CoV-2 (COVID-19) infections. Am J Physiol-Lung C. 2021 Jan 1; 320(1): L158–L163 IHC ; Human.
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| 研究領域 | 激酶和磷酸酶 合成與降解 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Rat (predicted: Mouse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 19/30/37 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human cathepsin L1 proprotein: 71-170/334 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene. Function: Important for the overall degradation of proteins in lysosomes. Subunit: Dimer of a heavy and a light chain linked by disulfide bonds. Subcellular Location: Lysosome. Similarity: Belongs to the peptidase C1 family. SWISS: P07711 Gene ID: 1514 Database links: Entrez Gene: 1514 Human Entrez Gene: 13039 Mouse Omim: 116880 Human SwissProt: P07711 Human SwissProt: P06797 Mouse Unigene: 726015 Human Unigene: 930 Mouse Unigene: 1294 Rat 合成與降解（Synthesis and Degradation） 組織蛋白酶L(cathepsin L CL)屬于木瓜蛋白酶家族中的半胱氨酸蛋白水解酶，以酶原的形式貯存于溶酶體中. 參與許多特殊的生理過程，如激素原的激活、抗原呈遞、組織器官的發(fā)育等。 在病理狀態(tài)下，因各種原因所致的細胞損傷(如病原微生物、炎癥因子、氧化應激等)可降解細胞成分或細胞間質基質成分，包括層粘素、IV型膠原纖維及纖維連接素等，與人類許多疾病如腫瘤的浸潤與轉移、關節(jié)炎、骨質疏松、阿爾茨海姆病、多發(fā)性硬化癥及其他慢性炎癥性疾病有關. |
| 產品圖片 |
Sample:
Lane 1: Human HUVEC cell Lysates
Lane 2: Human K562 cell Lysates
Lane 3: Human MCF-7 cell Lysates
Primary: Anti-Cathepsin L (bs-1508R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19/30/37kDa
Observed band size: 40kDa
Protein: line1, rat brain lysates, 30ug; line2, rat liver lysates, 30ug;
Primary: Anti-Cathepsin L (bs-1508R) at 1:200;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000;
ECL excitated the fluorescence;
Predicted band size : 37kD
Observed band size : 19kD, 37kD
Sample:
A549 Cell (Human) Lysate at 30 ug
K562 Cell (Human) Lysate at 30 ug
Primary: Anti- Cathepsin L (bs-1508R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19/30/37 kD
Observed band size: 40 kD
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cathepsin L) Polyclonal Antibody, Unconjugated (bs-1508R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cathepsin Polyclonal Antibody, Unconjugated(bs-1508R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cathepsin L Polyclonal Antibody, Unconjugated(bs-1508R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:A549.
Primary Antibody (green line): Rabbit Anti-Cathepsin L antibody (bs-1508R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
