| 產品編號 | bs-1684R |
| 英文名稱 | SKI Rabbit pAb |
| 中文名稱 | C-SKI抗體 |
| 別 名 | C-SKI; v-SKI; Sloan-kettering viral oncogene homolog; Avian sarcoma viral(v ski) oncogene homolog; C oncogene; C ski; Ski oncogene; Ski oncoprotein; SKV; v ski avian sarcoma viral oncogene homolog; v ski sarcoma viral oncogene homolog; v-ski sarcoma viral oncogene homolog; SKI_HUMAN. |
| 研究領域 | 腫瘤 轉錄調節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | (predicted: Human,Mouse,Rat,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 80 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human C-SKI: 551-650/728 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
SKI is a nuclear protein that forms homodimers and heterodimers and binds to DNA to function as transcriptional activators and repressors. The SKI oncoprotein dramatically affects cell growth, differentiation, and/or survival. SKI was shown to act in distinct signaling pathways including those involving nuclear receptors, transforming growth factor beta, and tumor suppressors. SKI inhibits transforming growth factor-beta (TGF-beta) signaling through interaction with Smad proteins. SKI represses Smad-mediated transcriptional activation, probably through its action as a transcriptional co-repressor. SKI also inhibits TGF-beta-induced downregulation of genes such as c-myc. Function: May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling. Subunit: Interacts with SMAD2, SMAD3 and SMAD4. Interacts with HIPK2. Part of a complex with HIPK2 and SMAD1/2/3. Interacts with PRDM16 and SMAD3; the interaction with PRDM16 promotes the recruitment SMAD3-HDAC1 complex on the promoter of TGF-beta target genes. Subcellular Location: Nucleus. Similarity: Belongs to the SKI family. SWISS: P12755 Gene ID: 6497 Database links: Entrez Gene: 6497 Human Entrez Gene: 20481 Mouse Omim: 164780 Human SwissProt: P49140 Chicken SwissProt: P12755 Human SwissProt: Q60698 Mouse Unigene: 656507 Human SKI又稱SKI、SKV，經研究發(fā)現SKI蛋白是刺激和誘發(fā)腫瘤生長導致癌癥的關鍵蛋白質之一，SKI蛋白主要通過阻止TGF-β蛋白發(fā)揮作用,當TGF-β蛋白的信號傳導被SKI蛋白阻斷后，細胞就可能加速增值、生長，最終導致癌癥發(fā)生。 有研究表明：c-ski可調節(jié)多種核因子的轉錄活性,參與神經系統發(fā)育、造血細胞的增殖和分化、腫瘤發(fā)生、組織再生等多種生理病理過程，更詳細的作用機制還在進一步研究之中。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
