| 產(chǎn)品編號 | bs-2122R |
| 英文名稱 | Rabbit Anti-GST tag antibody |
| 中文名稱 | GST標簽抗體 |
| 別 名 | GST PI protein; Glutathione S Transferase; Recombinant human Glutathione S Transferase pi protein; Glutathione S-transferase class-mu 26 kDa isozyme; GST; Sj26 antigen; SjGST; GST-tag. GST PI 蛋白; |
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Specific References (2) | bs-2122R has been referenced in 2 publications.
[IF=17.521] Qing Li. et al. SAMD9 Promotes Postoperative Recurrence of Esophageal Squamous Cell Carcinoma by Stimulating MYH9-Mediated GSK3β/β-Catenin Signaling. Advanced Science. 2023 Feb;:2203573 Pull Down,WB ; Human.
[IF=0] Cao, Meng, et al. "The formation of intracellular nanoparticles correlates with cisplatin resistance." Science China Materials (2015): 1-9. WB ;
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| 產(chǎn)品類型 | 標簽抗體 |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學 信號轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Recombinant protein |
| 產(chǎn)品應(yīng)用 | WB=1:20000-60000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant full length GST tag protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
GST is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. SWISS: N/A Gene ID: N/A Database links:
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| 產(chǎn)品圖片 |
Transformed (GST-tag or EV) E. coli cells lysates were subjected to SDS-PAGE followed by WB with bs-2122R (Anti-GST) at dilution of 1:60,000 incubated at 4℃ overnight.
Sample:
GST-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 4 ug
Primary: Anti-GST Tag (bs-2122R) at 1/1000 ~ 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
