| 產(chǎn)品編號 | bs-2735R |
| 英文名稱 | Rabbit Anti-GSTP1 antibody |
| 中文名稱 | 谷胱甘肽硫轉移酶pi抗體 |
| 別 名 | Glutathione S-Transferase pi; GST-Pi; DFN7; Gst-P1; FAEES3; Fatty Acid Ethyl Ester Synthase III; Glutathione S Transferase Pi; GST3; GSTP1; GST pi; PI; GSTP1_HUMAN. |
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Specific References (6) | bs-2735R has been referenced in 6 publications.
[IF=66.85] Fangfei Qin. et al. Linking chromatin acylation mark-defined proteome and genome in living cells. CELL. 2023 Mar;186:1066-1085.e36 Protein purification ;
[IF=7.83] Zhang, Chunlei, et al. "Insights into the Distinguishing Stress-induced Cytotoxicity of Chiral Gold Nanoclusters and the Relationship with GSTP1." Theranostics WB ; Human.
[IF=6.158] Jiang et al. Scavenger receptor-C acts as a receptor for Bacillus thuringiensis vegetative insecticidal protein Vip3Aa and mediates the internalization of Vip3Aa via endocytosis. (2018) PLoS.Pathog. 14:e1007347 WB ; Mouse.
[IF=3.15] Li, Hongyan, et al. "Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes." Toxicology Letters (2014). WB ; Mouse.
[IF=2.766] Lin et al. Penehyclidine Hydrochloride Preconditioning Provides Cardioprotection in a Rat Model of Myocardial Ischemia/Reperfusion Injury. (2015) PLoS.One. 10:e0138051 WB ; Rat.
[IF=2.66] Zhang, Fu, et al. "Up-regulation of protein tyrosine nitration in methamphetamine-induced neurotoxicity through DDAH/ADMA/NOS pathway." Neurochemistry International (2013). WB ; Rat.
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| 研究領域 | 腫瘤 細胞生物 免疫學 神經(jīng)生物學 轉錄調節(jié)因子 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg /test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 23kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GstP1: 121-210/210 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Glutathione S-transferases (GSTs) function in the metabolic detoxification of various environmental carcinogens and lipid hydroperoxides. Members of the murine GSTP (glutathione S-transferase pi) family, termed Gstp1 and Gstp2, are linked to drug resistance and are markers for many cancers. Gstp proteins modulate cell signaling by interacting with c-Jun N-terminal kinase (JNK), and may play a protective role in the development of spontaneous tumors. Gstp has been found in substantia nigra and may be associated with reactive oxygen species-induced neurological disorders such as Parkinson’s disease and may additionally protect against endothelial dysfunction induced by tobacco smoke exposure. Function: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Subunit: Homodimer. Tissue Specificity: Ubiquitously expressed. Similarity: Belongs to the GST superfamily. Pi family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain. SWISS: P19157 Gene ID: 2950 Database links: Entrez Gene: 2950 Human Omim: 134660 Human SwissProt: P09211 Human Unigene: 523836 Human GST-Pi是GST家族中的一種主要的同工酶，廣泛存在于全身器官中，尤以肝腎含量高，具有解毒功能。在多種腫瘤中高表達。目前認為是腫瘤細胞產(chǎn)生耐藥的一種標記，與腫瘤的耐藥（阿霉素、順鉑、氮芥、環(huán)磷酰胺和瘤可寧等）有關。該抗體主要用于細胞耐藥方面的研究。 |
| 產(chǎn)品圖片 |
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GSTP1 Polyclonal Antibody, Unconjugated(bs-2735R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: mouse splenocytes(blue)
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
