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產品編號	bs-3577R
英文名稱	DMT1 Rabbit pAb
中文名稱	金屬離子轉運體1抗體
別名	DCT 1; DCT1; Divalent metal transporter 1; DMT 1; Natural resistance associated macrophage protein 2; NRAMP 2; NRAMP2; SLC11A2; NRAM2_HUMAN.
	Specific References (8) \| bs-3577R has been referenced in 8 publications. [IF=10.787] Limin Shi. et al. FoxO1 regulates adipose transdifferentiation and iron influx by mediating Tgfβ1 signaling pathway. REDOX BIOL. 2023 Jul;63:102727 WB ; Mouse. PubMed:37156218 [IF=6.543] Zhang Hongxia. et al. Hepcidin Promoted Ferroptosis through Iron Metabolism which Is Associated with DMT1 Signaling Activation in Early Brain Injury following Subarachnoid Hemorrhage. Oxid Med Cell Longev. 2021;2021:9800794 WB,IF ; Rat. PubMed:34987706 [IF=6.17] Qian Wang. et al. Efficient iron utilization compensates for loss of extracellular matrix of ovarian cancer spheroids. Free Radical Bio Med. 2021 Feb;164:369 IF ; Human. PubMed:33450374 [IF=5.959] Zhang C et al. “Iron free” zinc oxide nanoparticles with ion-leaking properties disrupt intracellular ROS and iron homeostasis to induce ferroptosis. Cell Death Dis. 2020 Mar 13;11(3):183. WB ; mouse. PubMed:32170066 [IF=3.7] Zhang, Ying, et al. "Calcium channel blockers ameliorate iron overload-associated hepatic fibrosis by altering iron transport and stellate cell apoptosis." Toxicology and Applied Pharmacology (2016). WB ; Mouse. PubMed:27095094 [IF=3.378] Song, Yanqiu. et al. Iron overload impairs renal function and is associated with vascular calcification in rat aorta. BIOMETALS. 2022 Sep;:1-15 IF ; Rat. PubMed:36178540 [IF=2.1] Zhang, L. Y., et al. "Effect of iron source on iron absorption and gene expression of iron transporters in the ligated duodenal loops of broilers." Journal of Animal Science (2017). WB ; Chicken. PubMed:28464091 [IF=0] Lan B et al. Extract of Naotaifang, a compound Chinese herbal medicine, protects neuron ferroptosis induced by acute cerebral ischemia in rats. Journal of Integrative Medicine. 2020. IHC-P ; Rat. PubMed:doi:10.1016/j.joim.2020.01.008
研究領域	腫瘤免疫學神經生物學
抗體來源	Rabbit
克隆類型	Polyclonal
克隆號
交叉反應	Human,Mouse,Rat (predicted: Cow)
產品應用	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	62 kDa
檢測分子量
細胞定位	細胞漿
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human DMT1: 251-350/568
亞型	IgG
純化方法	affinity purified by Protein A
緩沖液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產品介紹	DMT1 is a major transporter of ferrous iron from the intestinal lumen. DMT1 belongs to the family of NRAMP metal ion transporters which function in diverse organisms from bacteria to human. DMT1 (NRAMP2) transports metal ions at the plasma membrane of cells of both the duodenum and in peripheral tissues, and defective DMT1 causes anaemia. Function: Actin-bundling protein. May function in mitogen-activated protein kinase pathway. Subcellular Location: Cytoplasm. Tissue Specificity: Heart, brain, lung, skeletal muscle, and kidney. Post-translational modifications: Actin-bundling activity is abolished upon phosphorylation by cAMP-dependent protein kinase. Similarity: Belongs to the villin/gelsolin family. Contains 1 HP (headpiece) domain. SWISS: P49281 Gene ID: 4891 Database links: Entrez Gene: 4891 Human Entrez Gene: 18174 Mouse Omim: 600523 Human SwissProt: P49281 Human SwissProt: P49282 Mouse Unigene: 505545 Human Unigene: 234608 Mouse
產品圖片	Sample: Adrenal glands (Mouse) Lysate at 40 ug Primary: Anti-DMT1 (bs-3577R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 64 kD Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Divalent metal transporter 1; DMT1) Polyclonal Antibody, Unconjugated (bs-3577R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Divalent metal transporter 1; DMT1) Polyclonal Antibody, Unconjugated (bs-3577R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DMT1) Polyclonal Antibody, Unconjugated (bs-3577R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

	免疫組化常用試劑
	免疫印跡常用試劑
	各種標記二抗

	1、抗體溶解方法
	2、抗體修復方式
	3、常用試劑的配制
	4、免疫組化操作步驟
	5、免疫組化問題解答
	6、Western Blotting 操作步驟
	7、Western Blotting 問題解答
	8、關于肽鏈的設計
	9、多肽的溶解與保存
	10、酶標抗體效價測定程序

	AbBy Fluor 350 標記
	AbBy Fluor 488 標記
	AbBy Fluor 555 標記
	AbBy Fluor 594 標記
	AbBy Fluor 647 標記
	AP 標記
	APC 標記
	Biotin 標記
	Cy3 標記
	Cy5 標記
	Cy5.5 標記
	Cy7 標記
	FITC 標記
	Gold 標記
	HRP 標記
	PE 標記
	PE-Cy3 標記
	PE-CY5 標記
	PE-CY5.5 標記
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	RBITC 標記