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ACADM Rabbit pAb (bs-4047R)  
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產(chǎn)品編號(hào) bs-4047R
英文名稱(chēng) ACADM Rabbit pAb
中文名稱(chēng) 酰基輔酶A脫氫酶中鏈抗體
別    名 mitochondrial antibody; ACAD 1; ACAD1; Acyl coenzyme A dehydrogenase; Acyl coenzyme A dehydrogenase C 4 to C 12 straight chain; MCAD; MCADH; Medium chain acyl CoA dehydrogenase; Medium chain fatty acyl CoA dehydrogenase; Medium chain specific acyl CoA dehydrogenase; Medium chain specific acyl CoA dehydrogenase mitochondrial; FLJ18227; FLJ93013; FLJ99884; ACADM_HUMAN.  
研究領(lǐng)域 腫瘤  細(xì)胞生物  免疫學(xué)  轉(zhuǎn)錄調(diào)節(jié)因子  線粒體  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 47 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞漿 線粒體
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ACADM: 151-250/421 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 ACADM protein is a medium chain specific (C4 to C12 straight chain) acyl Coenzyme A dehydrogenase. The enzyme catalyzes the initial step of the mitochondrial fatty acid beta oxidation pathway. ACADM expression is induced during periods of fasting, when reliance on fatty acids for energy is increased. Clinical phenotypes are associated with ACADM hereditary deficiency.

Function:
This enzyme is specific for acyl chain lengths of 4 to 16.

Subunit:
Homotetramer. Interacts with the heterodimeric electron transfer flavoprotein ETF.

Subcellular Location:
Mitochondrion matrix.

DISEASE:
Acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]: An inborn error of mitochondrial fatty acid beta-oxidation which causes fasting hypoglycemia, hepatic dysfunction and encephalopathy, often resulting in death in infancy. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the acyl-CoA dehydrogenase family.

SWISS:
P11310

Gene ID:
34

Database links:

Entrez Gene: 505968 Cow

Entrez Gene: 34 Human

Entrez Gene: 11364 Mouse

Entrez Gene: 24158 Rat

Omim: 607008 Human

SwissProt: Q3SZB4 Cow

SwissProt: P11310 Human

SwissProt: P45952 Mouse

SwissProt: P08503 Rat

Unigene: 445040 Human

Unigene: 10530 Mouse

Unigene: 6302 Rat



產(chǎn)品圖片
Sample: Lane 1: Mouse Liver tissue lysates Lane 2: Mouse Stomach tissue lysates Lane 3: Mouse Heart tissue lysates Lane 4: Rat Heart tissue lysates Lane 5: Human K562 cell lysates Lane 6: Human Hela cell lysates Lane 7: Human THP-1 cell lysates Primary: Anti-ACADM (bs-4047R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ACADM) Polyclonal Antibody, Unconjugated (bs-4047R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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