| 產(chǎn)品編號 | bs-7931R |
| 英文名稱 | PPP2R1A Rabbit pAb |
| 中文名稱 | 蛋白質磷酸酶2調節(jié)亞基1A抗體 |
| 別 名 | PP2A; PP2A subunit A PR65-alpha isoform; PP2A subunit A R1 alpha isoform; PP2A, subunit A, PR65-alpha isoform; PP2A, subunit A, R1-alpha isoform; PPP2R1A; ppp2r1a-b; PR65; PR65A; Protein phosphatase 2(formerly 2A), regulatory subunit A(PR 65), alpha isoform; Protein phosphatase 2(Formerly 2A), regulatory subunit A(PR 65), alpha isoform, isoform CRA_a; Protein phosphatase 2(formerly 2A), regulatory subunit A, ; Protein phosphatase 2 regulatory subunit A alpha isoform; Protein phosphatase 2, 65-KD regulatory subunit A, alpha; Protein phosphatase 2, regulatory subunit A, alpha isoform; Protein phosphatase 2, structural/regulatory subunit A, alpha; PPP2R1A; Serine threonine protein phosphatase 65 kDa; Serine/threonine protein phosphatase 2A 65 KDA regulatory subunit A alpha isoform; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; alpha isoform of regulatory subunit A, protein phosphatase; DKFZp470L0914; Medium tumor antigen-associated 61 kDa protein; 2AAA_HUMAN. |
| 研究領域 | 細胞生物 免疫學 信號轉導 細胞周期蛋白 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse (predicted: Rat,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 65 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PPP2R1A: 166-255/589 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Protein phosphatase 2A (PP2A) is the most abundant phosphatase specific for serine and threonine phosphorylation in mammalian cells. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit alpha (PPP2R1A) serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory beta subunit. Function: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis. Subunit: Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD (By similarity). PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with TP53 and SGOL1. Interacts with PLA2G16; this interaction might decrease PP2A activity. Subcellular Location: Cytoplasm. Chromosome, centromere. Similarity: Belongs to the phosphatase 2A regulatory subunit A family. Contains 15 HEAT repeats. SWISS: P30153 Gene ID: 5518 Database links: ntrez Gene: 5518 Human Entrez Gene: 51792 Mouse Omim: 605983 Human SwissProt: P30153 Human SwissProt: Q76MZ3 Mouse Unigene: 467192 Human Unigene: 294138 Mouse Unigene: 4108 Rat
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| 產(chǎn)品圖片 |
Sample:
Hela(Human) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-PPP2R1A (bs-7931R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
