| 產(chǎn)品編號(hào) | bs-8390R |
| 英文名稱 | SHFM3 Rabbit pAb |
| 中文名稱 | SHFM3蛋白抗體 |
| 別 名 | DAC; Dactylin; F box and WD 40 domain containing protein 4; F box and WD 40 domain protein 4; F box and WD repeat domain containing 4; F box/WD repeat containing protein 4; F box/WD repeat protein 4; F-box and WD-40 domain-containing protein 4; F-box/WD repeat-containing protein 4; FBW 4; FBW4; FBWD 4; FBWD4; FBXW 4; FBXW4; FBXW4_HUMAN; SHFM 3; SHSF 3; SHSF3; Split hand/foot malformation(ectrodactyly) type. |
| 研究領(lǐng)域 | 細(xì)胞生物 發(fā)育生物學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 46 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SHFM3: 171-270/412 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. Likely to be involved in key signaling pathways crucial for normal limb development. May participate in Wnt signaling. Involvement in disease:Defects in FBXW4 are a cause of split-hand/foot malformation type 3 (SHFM3) . SHFM3 is an autosomal dominant disorder characterized by hypoplasia/aplasia of the central digits with fusion of the remaining digits. Function: Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. Likely to be involved in key signaling pathways crucial for normal limb development. May participate in Wnt signaling. Subunit: Part of a SCF (SKP1-cullin-F-box) protein ligase complex (By similarity). Subcellular Location: Expressed in brain, kidney, lung and liver. Tissue Specificity: Expressed in brain, kidney, lung and liver. DISEASE: Defects in FBXW4 are a cause of split-hand/foot malformation type 3 (SHFM3) [MIM:246560]. SHFM3 is an autosomal dominant disorder characterized by hypoplasia/aplasia of the central digits with fusion of the remaining digits. Similarity: Contains 1 F-box domain. Contains 4 WD repeats. SWISS: P57775 Gene ID: 6468 Database links: Entrez Gene: 6468 Human Omim: 608071 Human SwissProt: P57775 Human Unigene: 500822 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SHFM3) Polyclonal Antibody, Unconjugated (bs-8390R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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