| 產品編號 | bs-7357R |
| 英文名稱 | TOMM20 Rabbit pAb |
| 中文名稱 | 線粒體外膜受體Tom20抗體 |
| 別 名 | TOM20; KIAA0016; MAS20; MGC117367; Mitochondrial 20 kDa outer membrane protein; Mitochondrial import receptor subunit TOM20 homolog; MOM19; Outer mitochondrial membrane receptor Tom20; TOM 20; TOM20_HUMAN; Translocase of outer mitochondrial membrane 20 homolog(yeast); Translocase of outer mitochondrial membrane 20 homolog type II. |
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Specific References (1) | bs-7357R has been referenced in 1 publications.
[IF=6.02] Zhang J et al. Disruption of the Superoxide Anions-Mitophagy Regulation Axis Mediates Copper Oxide Nanoparticles-Induced Vascular Endothelial Cell Death. Free Radic. Biol. Med. Sep 21;129:268-278 WB ; Human.
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| 研究領域 | 腫瘤 細胞生物 信號轉導 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 16 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TOM20: 1-100/145 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The mitochondrial preprotein translocases of the outer membrane (Tom) is a multisubunit protein complex that facilitates the import of nucleus-encoded precursor proteins across the mitochondrial outer membrane (1). The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria (2). The import receptors include Tom20 and Tom22, which form a heteromeric receptor complex that initiates the insertion of newly synthesized proteins into the outer membrane and then directs the precursor protein into the GIP (3,4). In yeast, Tom22 is the essential component of the import receptor complex as it functions as both a receptor for the preproteins and serves as a docking point for both Tom20 and the GIP (5,6). Tom22 directly associates with Tom40, the major component of the GIP, and thereby forms a stable interaction between the two core complexes to facilitate the fluid movement of preproteins into the mitochondria (6,7). The insertion of Tom40 into the Tom machinery requires the initial binding of Tom40 to Tom20 and leads to the efficient incorporation of Tom40 precursors into preexisting Tom complexes (2,8) Function: Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. Subunit: Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex). Subcellular Location: Mitochondrion outer membrane. Similarity: Belongs to the Tom20 family. SWISS: Q15388 Gene ID: 9804 Database links: Entrez Gene: 9804 Human Entrez Gene: 67952 Mouse SwissProt: Q15388 Human SwissProt: Q9DCC8 Mouse |
| 產品圖片 |
Sample:
Lane 1: Mouse Kidney Lysates
Lane 2: Mouse Brain Lysates
Lane 3: Mouse Heart Lysates
Lane 4: Mouse NIH/3T3 Lysates
Lane 5: Rat Kidney Lysates
Lane 6: Rat Brain Lysates
Lane 7: Rat Heart Lysates
Lane 8: Human Hela cell Lysates
Lane 9: Human THP-1 cell Lysates
Lane 10: Human HL-60 cell Lysates
Lane 11: Human 293T cell Lysates
Primary: Anti-TOMM20 (bs-7357R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 16kDa
Observed band size: 16kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
