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TOMM20 Rabbit pAb (bs-7357R)  
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產品編號 bs-7357R
英文名稱 TOMM20 Rabbit pAb
中文名稱 線粒體外膜受體Tom20抗體
別    名 TOM20; KIAA0016; MAS20; MGC117367; Mitochondrial 20 kDa outer membrane protein; Mitochondrial import receptor subunit TOM20 homolog; MOM19; Outer mitochondrial membrane receptor Tom20; TOM 20; TOM20_HUMAN; Translocase of outer mitochondrial membrane 20 homolog(yeast); Translocase of outer mitochondrial membrane 20 homolog type II.  
Specific References  (1)     |     bs-7357R has been referenced in 1 publications.
[IF=6.02] Zhang J et al. Disruption of the Superoxide Anions-Mitophagy Regulation Axis Mediates Copper Oxide Nanoparticles-Induced Vascular Endothelial Cell Death. Free Radic. Biol. Med. Sep 21;129:268-278  WB ;  Human.  
研究領域 腫瘤  細胞生物  信號轉導  線粒體  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 16 kDa
檢測分子量
細胞定位 細胞漿 線粒體
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human TOM20: 1-100/145 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The mitochondrial preprotein translocases of the outer membrane (Tom) is a multisubunit protein complex that facilitates the import of nucleus-encoded precursor proteins across the mitochondrial outer membrane (1). The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria (2). The import receptors include Tom20 and Tom22, which form a heteromeric receptor complex that initiates the insertion of newly synthesized proteins into the outer membrane and then directs the precursor protein into the GIP (3,4). In yeast, Tom22 is the essential component of the import receptor complex as it functions as both a receptor for the preproteins and serves as a docking point for both Tom20 and the GIP (5,6). Tom22 directly associates with Tom40, the major component of the GIP, and thereby forms a stable interaction between the two core complexes to facilitate the fluid movement of preproteins into the mitochondria (6,7). The insertion of Tom40 into the Tom machinery requires the initial binding of Tom40 to Tom20 and leads to the efficient incorporation of Tom40 precursors into preexisting Tom complexes (2,8)

Function:
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.

Subunit:
Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex).

Subcellular Location:
Mitochondrion outer membrane.

Similarity:
Belongs to the Tom20 family.

SWISS:
Q15388

Gene ID:
9804

Database links:
Entrez Gene: 9804 Human

Entrez Gene: 67952 Mouse

Entrez Gene: 266601 Rat

SwissProt: Q15388 Human

SwissProt: Q9DCC8 Mouse

SwissProt: Q62760 Rat



產品圖片
Sample: Lane 1: Mouse Kidney Lysates Lane 2: Mouse Brain Lysates Lane 3: Mouse Heart Lysates Lane 4: Mouse NIH/3T3 Lysates Lane 5: Rat Kidney Lysates Lane 6: Rat Brain Lysates Lane 7: Rat Heart Lysates Lane 8: Human Hela cell Lysates Lane 9: Human THP-1 cell Lysates Lane 10: Human HL-60 cell Lysates Lane 11: Human 293T cell Lysates Primary: Anti-TOMM20 (bs-7357R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 16kDa Observed band size: 16kDa
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