產(chǎn)品編號 | bs-18048R |
英文名稱 | HMCN2 Rabbit pAb |
中文名稱 | 半椎蛋白2抗體 |
別 名 | Hemicentin 2 |
研究領域 | 細胞生物 免疫學 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
克 隆 號 | |
交叉反應 | (predicted: Human,Mouse,Sheep,Cow,Dog,Horse) |
產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 540 kDa |
檢測分子量 | |
細胞定位 | 分泌型蛋白 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human HMCN2: 101-200/5065 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
First discovered in C. elegans, Hemicentins are extracellular matrix (ECM) proteins that contain a single von Willebrand A domain at the amino terminus, more than 40 tandem immunoglobulin domains, multiple tandem epidermal growth factors, and a single fibulin-like carboxy-terminal module. In mammals, Hemicentins are expressed in the ECM of eye, skin and tongue epithelium and in the ECM of some blood vessels. Due to the expression pattern in areas that are subject to a significant amount of stress, it is thought that Hemicentins likely play a role in the architecture of flexible and adhesive cell junctions. Hemicentin-2 is a 5,065 amino acid secreted protein that is expressed in the extracellular matrix. There are three isoforms of Hemicentin-2 that are produced as a result of alternative splicing events Subcellular Location: Secreted Similarity: Contains 5 EGF-like domains. Contains 42 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 nidogen G2 beta-barrel domain. Contains 1 VWFA domain. SWISS: Q8NDA2 Gene ID: 256158 Database links: Entrez Gene: 256158 Human SwissProt: Q8NDA2 Human |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |