| 產(chǎn)品編號 | bs-48026P |
| 英文名稱 | Recombinant human IL-4 protein (Active, HEK293) |
| 中文名稱 | 重組人IL-4 (活性的, Tag free) |
| 別 名 | rhIL-4; IL4; B cell growth factor 1; B cell IgG differentiation factor; B Cell Stimulatory Factor 1; BCGF 1; BCGF1; Binetrakin; BSF 1; BSF1; HCGF; Hodgkin’s Cell Growth Factor; IA Inducing Factor; IGG1 induction factor; IL 4; Il4e12; Interleukin 4; Interleukin 4, isoform 1; Interleukin4; Lymphocyte stimulatory factor 1; Macrophage fusion factor; Mast cell growth factor 2; MCGF2; MFF; MGC79402; Pitrakinra; T cell growth factor 2; TCGF2; IL4_MOUSE; Interleukin-4; IL-4; B-cell stimulatory factor 1; BSF-1. ;Recombinant human IL-4 protein (Active, HEK293) 人IL-4重組蛋白; 重組人IL-4; 人重組IL-4; 重組人IL-4; 白細胞介素4; 白介素4; |
| 克 隆 號 | |
| 理論分子量 | 14-19 kDa |
| 檢測分子量 | |
| 性 狀 | Lyophilized |
| 濃 度 | ＞0.5 mg/ml |
| 物 種 | Human |
| 序 列 | 25-153/153 |
| 純 度 | >95% as determined by SDS-PAGE |
| 純化方法 | AC |
| 內毒素 | <0.5 EU/μg as determined by LAL. |
| 表達系統(tǒng) | HEK293 cell |
| 活性 | Yes |
| 標簽 | Tag free |
| 緩 沖 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存條件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after r |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
Interleukin 4 (IL-4) is a 14-19kDa glycosylated monomer, belongs to the IL-4 / IL-13 family. IL-4 is primarily expressed by Th2biased CD4+ T cells, mast cells, basophils, and eosinophils. IL-4 is a pleiotropic cytokine that participates in at least several B-cell activation processes as well as of other cell types. It is a co-stimulator of DNAsynthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. This recombinant human IL-4 is produced by human cells. (Tag free) Biological activity: The activity was measured by its ability to stimulate the proliferation of human TF-1 cells (human erythroleukemic indicator cell line). Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. SWISS: P05112 Gene ID: 3565 |
| 產(chǎn)品圖片 |
The purity of the protein is greater than 95% as determined by reduced and non reduced SDS-PAGE.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
