| 產(chǎn)品編號 | bsm-60560R |
| 英文名稱 | Fibrillarin Recombinant Rabbit mAb |
| 中文名稱 | |
| 別 名 | 34 kD nucleolar scleroderma antigen; 34 kDa nucleolar scleroderma antigen; 34-kD nucleolar scleroderma antigen; FBL; FBRL_HUMAN; FIB; FIB1; FLRN; Nop1p; RNU3IP1; rRNA 2' O methyltransferase fibrillarin; rRNA 2''-O-methyltransferase fibrillarin; rRNA 2'-O-methyltransferase fibrillarin. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | H4C3 |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-2000,Flow-Cyt=1:50-100,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG/Kappa |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | S-腺苷-L-甲硫氨酸依賴的甲基轉移酶，具有使RNA和蛋白質(zhì)甲基化的能力。通過催化前核糖體RNA中核糖部分的位點特異性2'-羥基甲基化參與前rRNA加工。位點特異性由與底物堿基配對的引導RNA提供。甲基化發(fā)生在與引導RNA堿基配對的序列的特征距離處。可能催化C/D-RNP復合物中U6-snRNAs的2'-O-甲基化。U6-snRNA 2'-O-甲基化是mRNA剪接保真度所必需的。還通過介導組蛋白H2A（H2AQ104me）的“Gln-105”甲基化而充當?shù)鞍踪|(zhì)甲基轉移酶，這是一種損害FACT復合物結合的修飾，并特異性存在于35S核糖體DNA位點。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Fibrillarin monoclonal antibody, unconjugated (bsm-60560R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (FBL) monoclonal Antibody, unconjugated (bsm-60560R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-FBL antibody (bsm-60560R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488(bs-60295G-BF488): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
