| 產(chǎn)品編號 | bsm-60573R |
| 英文名稱 | RBBP4 Recombinant Rabbit mAb |
| 中文名稱 | 視網(wǎng)膜母細胞瘤結(jié)合蛋白P48重組兔單抗 |
| 別 名 | RBBP4_HUMAN; Histone-binding protein RBBP4; Chromatin assembly factor 1 subunit C; CAF-1 subunit C; Chromatin assembly factor I p48 subunit; CAF-I 48 kDa subunit; CAF-I p48; Nucleosome-remodeling factor subunit RBAP48; Retinoblastoma-binding protein 4; RBBP-4; Retinoblastoma-binding protein p48; RbAp48. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | G7A9 |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:1000,IHC-P=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 47 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG/Kappa |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008] 核心組蛋白結(jié)合亞單位，可靶向染色質(zhì)組裝因子、染色質(zhì)重塑因子和組蛋白脫乙酰基酶，其方式由核小體DNA調(diào)節(jié)。是幾種調(diào)節(jié)染色質(zhì)代謝的復(fù)合物的成分。這些包括染色質(zhì)組裝因子1（CAF-1）復(fù)合物，它是DNA復(fù)制和DNA修復(fù)后染色質(zhì)組裝所必需的。核心組蛋白去乙?；福℉DAC）復(fù)合物，促進組蛋白去乙酰化和隨后的轉(zhuǎn)錄抑制，核小體重構(gòu)和組蛋白去乙酰化酶復(fù)合物（NuRD復(fù)合物），通過組蛋白去乙?；秃诵◇w重構(gòu)促進轉(zhuǎn)錄抑制。PRC2/EED-EZH2復(fù)合物，在發(fā)育過程中促進同源異型基因的抑制。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: HeLa,HEK- 293, A20, Rat spleen
Protein loading quantity: 20 μg
Exposure time: 3 s
Predicted MW: 52 kDa
Observed MW: 52 kDa
Tissue: Human lung squamous carcinoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60573R
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
