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Crotonyl-Histone H2B (Lys11) Recombinant Rabbit mAb (bsm-60207R)  
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25ul/800.00元
50ul/1400.00元
100ul/2500.00元
大包裝/詢價

產(chǎn)品編號 bsm-60207R
英文名稱 Crotonyl-Histone H2B (Lys11) Recombinant Rabbit mAb
中文名稱
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 A10A2
交叉反應 Human,Mouse,Rat
產(chǎn)品應用 WB=1:500-1:2000,IHC-P=1:100-500,IHC-F=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 組蛋白經(jīng)過各種酶催化的修飾,包括乙?;?,甲基化,磷酸化,泛素化等。賴氨酸巴豆?;切掳l(fā)現(xiàn)的一種控制染色體結構和基因轉錄的可逆修飾。賴氨酸巴豆酰化在從蠕蟲到人的真核細胞中高度保守。組蛋白賴氨酸巴豆酰化獨特的結構和基因組定位表明,它在機制和功能上不同于組蛋白賴氨酸乙?;>唧w地說,在人類體細胞和兔雄性生殖細胞基因組中,組蛋白巴豆?;瘶酥局钚詥幼踊驖撛谠鰪娮印=M蛋白H2B在Lys11位點的巴豆?;赡茉诒碛^遺傳調控中發(fā)揮重要作用,包括染色質重塑和DNA轉錄調控等。
產(chǎn)品圖片
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: NIH-3T3, 3: BRL, 4: Rat kidney, 5: Mouse kidney Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 14 kDa Observed MW: 14 kDa
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-) HeLa, (+) HeLa+ crotonic acid (100 ng/ml, 18hr) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 14 kDa Observed MW: 14 kDa
Tissue: Human breast Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60207R
Tissue: Mouse small intestine Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60207R
Tissue: Rat liver Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60207R
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