| 產(chǎn)品編號(hào) | bsm-60784M |
| 英文名稱 | Nestin Mouse mAb |
| 中文名稱 | 巢蛋白/神經(jīng)上皮干細(xì)胞蛋白單克隆抗體 |
| 別 名 | ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_HUMAN; Nestin. |
| 抗體來(lái)源 | Mouse |
| 克隆類(lèi)型 | Monoclonal |
| 克 隆 號(hào) | E2G4 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-1000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 178 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nestin |
| 亞 型 | IgG2a, κ |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | PBS, Glycerol, BSA. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. Function: Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. Subunit: Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3. Subcellular Location: cytoplasm Tissue Specificity: CNS stem cells. Post-translational modifications: Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. Similarity: Belongs to the intermediate filament family. SWISS: P48681 Gene ID: 10763 Database links: Entrez Gene: 10763 Human Entrez Gene: 18008 Mouse Omim: 600915 Human SwissProt: P48681 Human SwissProt: Q6P5H2 Mouse Unigene: 527971 Human Unigene: 331129 Mouse Unigene: 9701 Rat 神經(jīng)干細(xì)胞標(biāo)志物在動(dòng)物體早期發(fā)育過(guò)程中，中樞神經(jīng)系統(tǒng)發(fā)育尤為重要 。Nestin是一種中等纖維蛋白，它在哺乳動(dòng)物神經(jīng)前體細(xì)胞中表達(dá)較高，目前已被廣泛用于神經(jīng)前體細(xì)胞的重要標(biāo)志分子。研究Nestin的表達(dá)調(diào)控規(guī)律，將有助于人們認(rèn)識(shí)神經(jīng)前體細(xì)胞的生物特性。此抗體還表達(dá)中樞神經(jīng)系統(tǒng)干細(xì)胞及神經(jīng)上皮細(xì)胞。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Nestin monoclonal antibody, unconjugated (bsm-60784M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Nestin monoclonal antibody, unconjugated (bsm-60784M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (human kidney ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Nestin) Monoclonal Antibody, Unconjugated (bsm-60784M) at 1:500 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human spleen ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Nestin) Monoclonal Antibody, Unconjugated (bsm-60784M) at 1:500 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Nestin) Monoclonal Antibody, Unconjugated (bsm-60784M) at 1:500 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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