| 產(chǎn)品編號 | bsm-43010M |
| 英文名稱 | MPO Mouse mAb |
| 中文名稱 | 髓過氧化物酶單克隆抗體 |
| 別 名 | Myeloperoxidase; c-ANCA; 89 kDa myeloperoxidase; 84 kDa yeloperoxidase; Myeloperoxidase light chain; Myeloperoxidase heavy chain; EC 1.11.1.7; PERM_HUMAN. |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 6D9 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 84 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 免 疫 原 | Recombinant human MPO protein |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014] Function: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Subunit: Tetramer of two light chains and two heavy chains. Subcellular Location: Lysosome. DISEASE: Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. Similarity: Belongs to the peroxidase family. XPO subfamily. SWISS: P05164 Gene ID: 4353 Database links: Entrez Gene: 4353 Human Entrez Gene: 17523 Mouse Omim: 606989 Human SwissProt: P05164 Human SwissProt: P11247 Mouse Unigene: 458272 Human Unigene: 4668 Mouse Unigene: 47782 Rat 髓過氧化物酶MPO,作為一種白細胞酶,具有介導(dǎo)炎性反應(yīng)、調(diào)節(jié)免疫應(yīng)答等多種功能,并可參與疾病的發(fā)生發(fā)展過程。同時,髓過氧化物酶基因存在基因多態(tài)性,也影響機體對疾病的易感性. 在正常淋巴組織中和各種髓樣細胞增生癥中，MPO均有較強表達，如：淋巴樣細胞、原核細胞、肥大細胞、漿細胞以及各種上皮源性腫瘤和肉瘤等。 髓過氧化物酶(myeloperoxidase,MPO),是一種血紅素蛋白，富含于中性粒細胞中,由粒細胞進入循環(huán)之前在骨髓內(nèi)合成并存儲于噬天青顆粒內(nèi)。外界刺激可導(dǎo)致中性粒細胞聚集，從而釋放髓過氧化物酶。MPO的相對分子量為150kDa，是由兩個亞單位通過共價結(jié)合形成的四聚體，每個亞單位又有一條重鏈α（相對分子量60kDa）和一條輕鏈β鏈（相對分子量為15kDa)構(gòu)成。（重鏈和輕鏈通過二硫鍵結(jié)合） |
| 產(chǎn)品圖片 |
50 ng rhMPO protein, C-His (HEK293) (bs-43010P) per lane probed with MPO monoclonal antibody respectively, unconjugated (bsm-43010M) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Gastric cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MPO Monoclonal Antibody, Unconjugated(bsm-43010M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsils; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MPO Monoclonal Antibody, Unconjugated(bsm-43010M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MPO Monoclonal Antibody, Unconjugated(bsm-43010M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
