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Anti-CD20 & CD47 Reference Antibody (Amulirafusp alfa Biosimilar) (BIO1012SM)  
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產(chǎn)品編號(hào) BIO1012SM
英文名稱 Anti-CD20 & CD47 Reference Antibody (Amulirafusp alfa Biosimilar)
別    名 CD20 & CD47; Amulirafusp alfa  
抗體來源
克隆類型 Monoclonal
交叉反應(yīng) Human
產(chǎn)品應(yīng)用
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 174.12kDa
性    狀 Lyophilized
亞    型 IgG-VHH
純化方法 Protein A
緩 沖 液 100 mM Pro-Ac 20mM Arg pH 5.0
保存條件 -80°C for 2 years under sterile conditions; -20°C for 1 year under sterile conditions;Avoid repeated freeze-thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹
產(chǎn)品圖片
Co-incubation of Imm0306 with Raji cell and PBMCs for 4 hours, then LDH was detected to evaluate the ADCC activity of Imm0306. As shown in fig, Imm0306 has ADCC activity, and the IC50 was 0.001 nM.
Imm0306 bound to Raji cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Imm0306 bound to Raji cells, and the EC50 was 1.438 nM.
Imm0306 bound to CCRF-CEM cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Imm0306 bound to CCRF-CEM.
Imm0306 bound to CD47 protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Imm0306 bound human CD47 Protein-His, and the EC50 was 0.015 nM.
Imm0306 bound to CD20 protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Imm0306 bound human CD20 VLP, and the EC50 was 0.056 nM.
The purity of Anti-CD20 & CD47 Reference Antibody (Imm0306) is 99.17%, determined by SEC-HPLC.
Anti-CD20 & CD47 Reference Antibody (Imm0306) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The detected molecular weight of Anti-CD20 & CD47 Reference Antibody (Amulirafusp alfa) is 173.84 kDa.
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