| 產(chǎn)品編號(hào) | bs-0558R |
| 英文名稱(chēng) | EMP-1 Rabbit pAb |
| 中文名稱(chēng) | 表皮膜蛋白1抗體 |
| 別 名 | CL-20; EMP 1; EMP1; EMP1_HUMAN; Epithelial membrane protein 1; Protein B4B; TMP; Tumor-associated membrane protein. |
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Specific References (1) | bs-0558R has been referenced in 1 publications.
[IF=20.722] Shaojun Peng. et al. Second near-infrared photoactivatable hydrogen selenide nanogenerators for metastasis-inhibited cancer therapy. Nano Today. 2021 Oct;40:101240 IF ; Mouse.
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| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 細(xì)胞凋亡 細(xì)胞膜受體 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 17 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse EMP-1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Epithelial membrane protein-1 (EMP-1) is a four pass transmembrane protein consisting of 160 amino acids. It is a member of a small family of epithelial membrane proteins. EMP-1 is very similar in structure to its close relative, Peripheral Myelin Protein 22 (PMP22). It is most predominantly expressed in tissues of the gastrointestinal tract but has also been found to be a junctional protein in the liver expressed along the intercellular border. EMP-1 directly interacts with the C-terminus of the P2X7 receptor and may be involved in membrane blebbing. EMP-1 may also be an important regulator in cell communication, signaling, and adhesion. When EMP-1 is overexpressed, cell proliferation is inhibited, S phase is arrested and G1 phase is prolonged in esophogeal cancer cells. EMP-1 may play a role in tumorigenesis and has been identified as a biomarker for gefitinib treatment resistance for patients with lung cancer. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Most prominently found in the gastrointestinal tract, skin, lung, and brain but not in liver. Similarity: Belongs to the PMP-22/EMP/MP20 family. Database links: Entrez Gene: 2012 Human Omim: 602333 Human SwissProt: P54849 Human Unigene: 719042 Human emp-1蛋白主要參與細(xì)胞生長(zhǎng)發(fā)育、細(xì)胞增殖、死亡、表皮發(fā)育等作用。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with EMP-1 polyclonal antibody, unconjugated (bs-0558R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EMP-1) Polyclonal Antibody, Unconjugated (bs-0558R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EMP-1) Polyclonal Antibody, Unconjugated (bs-0558R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EMP-1) Polyclonal Antibody, Unconjugated (bs-0558R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EMP-1) Polyclonal Antibody, Unconjugated (bs-0558R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EMP-1) Polyclonal Antibody, Unconjugated (bs-0558R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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