| 產(chǎn)品編號 | bs-7932R |
| 英文名稱 | PPP2R1B Rabbit pAb |
| 中文名稱 | 蛋白質(zhì)磷酸酶2調(diào)節(jié)亞基1B抗體 |
| 別 名 | 2AAB_HUMAN; beta isoform of regulatory subunit A, protein phosphatase 2; PP2A A beta; PP2A subunit A isoform PR65-beta; PP2A subunit A isoform R1-beta; PP2A, subunit A, PR65 beta isoform; PP2A, subunit A, R1 beta isoform; PPP2R1B; PR65B; protein phosphatase 2(formerly 2A), regulatory subunit A(PR 65), beta isoform; protein phosphatase 2(formerly 2A), regulatory subunit A, beta isoform; Protein phosphatase 2 regulatory subunit A alpha isoform; protein phosphatase 2, structural/regulatory subunit A, beta isoform; Serine threonine protein phosphatase 65 kDa; serine/threonine protein phosphatase 2A 65 kDa regulatory subunit A, beta isoform; serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, beta isoform; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform. |
| 研究領域 | 細胞生物 免疫學 信號轉(zhuǎn)導 細胞周期蛋白 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 65 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PPP2R1B: 21-120/601 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Function: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Subunit: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with SGOL1. Interacts with RAF1. Similarity: Belongs to the phosphatase 2A regulatory subunit A family. Contains 15 HEAT repeats. SWISS: P30154 Gene ID: 5519 Database links: Entrez Gene: 5519 Human Entrez Gene: 73699 Mouse Omim: 603113 Human SwissProt: P30154 Human SwissProt: Q7TNP2 Mouse Unigene: 584790 Human Unigene: 7726 Mouse Unigene: 163017 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPP2R1B) Polyclonal Antibody, Unconjugated (bs-7932R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
