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Human TNF-α ELISA Kit (bsk11014)

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產(chǎn)品信息

產(chǎn)品編號	bsk11014
英文名稱	Human TNF-α ELISA Kit
中文名稱	人腫瘤壞死因子α酶聯(lián)免疫試劑盒
別名	TNF-alpha; APC1; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; MCF; Necrosin; TNF-a; TNF alpha; TNF; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precursor; Tumour Necrosis Factor Alpha; TNFSF1A; TNFA_HUMAN.
	Specific References (10) \| bsk11014 has been referenced in 10 publications. [IF=20.693] Hanlin He. et al. ADAM9: A regulator between HCMV infection and function of smooth muscle cells. J MED VIROL. 2022 Nov;: ELISA ; Human. PubMed:36437481 [IF=12.479] Qi Chen. et al. Ruthenium complexes boost NK cell immunotherapy via sensitizing triple-negative breast cancer and shaping immuno-microenvironment. Biomaterials. 2022 Jan;:121371 ELISA ; Human. PubMed:35063740 [IF=8.079] Qiu, Zhenhua. et al. Human umbilical cord mesenchymal stem cell-derived exosomal miR-335-5p attenuates the inflammation and tubular epithelial–myofibroblast transdifferentiation of renal tubular epithelial cells by reducing ADAM19 protein levels. STEM CELL RES THER. 2022 Dec;13(1):1-15 WB ; Human. PubMed:35902972 [IF=5.999] Cheng Chen. et al. Effects of substrate topography on the regulation of human fibroblasts and capsule formation via modulating macrophage polarization. COLLOID SURFACE B. 2023 Feb;222:113086 ELISA ; Human. PubMed:36542953 [IF=4.964] Qing Zhu. et al. Soluble Vascular Cell Adhesion Molecule-1 as an Inflammation-Related Biomarker of Coronary Slow Flow. J CLIN MED. 2023 Jan;12(2):543 ELISA ; Human. PubMed:36675472 [IF=4.545] Feng Li. et al. Eriobotrya japonica leaf triterpenoid acids ameliorate metabolic syndrome in C57BL/6J mice fed with high-fat diet. Biomed Pharmacother. 2020 Dec;132:110866 ELISA ; PubMed:33113426 [IF=3.269] Meizhu Chen. et al. Helicobacter pylori promotes inflammatory factor secretion and lung injury through VacA exotoxin-mediated activation of NF-κB signaling. BIOENGINEERED. 2022;13(5):12760-12771 ELISA ; Human. PubMed:35603777 [IF=2.836] Dai Jiangwen. et al. Liver kinase B1 attenuates liver ischemia/reperfusion injury via inhibiting the NLRP3 inflammasome. Acta Bioch Bioph Sin. 2021 Mar;: ELISA ; Mouse. PubMed:33783473 [IF=2.493] Rifeng Chen. et al. Overexpression of CD86 enhances the ability of THP-1 macrophages to defend against Talaromyces marneffei. IMMUN INFLAMM DIS. 2022 Nov;10(12):e740 ELISA ; Human. PubMed:36444627 [IF=2.293] Qiu M et al. Effects of miR-150-5p on the growth and SOCS1 expression of rheumatoid arthritis synovial fibroblasts. Clin Rheumatol. 2019 Dec 26. ELISA ; Human. PubMed:31879859
克隆號
種屬	Human
線性范圍	15.60 - 1,000 pg/mL
應(yīng)用范圍	S/P/CC
檢測限	7 pg/mL
適用樣品基質(zhì)	cell culture supernates, serum, and plasma.
保存條件	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.).
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹	Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

	1、抗體溶解方法
	2、抗體修復(fù)方式
	3、常用試劑的配制
	4、免疫組化操作步驟
	5、免疫組化問題解答
	6、Western Blotting 操作步驟
	7、Western Blotting 問題解答
	8、關(guān)于肽鏈的設(shè)計
	9、多肽的溶解與保存
	10、酶標抗體效價測定程序

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