| 產(chǎn)品編號 | bsm-51615M |
| 英文名稱 | RAB1B Mouse mAb |
| 中文名稱 | RAB1B單克隆抗體 |
| 別 名 | RAB1B member RAS oncogene family; RAB1B_HUMAN; Ras related protein Rab1B; Ras-related protein Rab-1B; Small GTP binding protein. |
| 研究領(lǐng)域 | 信號轉(zhuǎn)導 G蛋白信號 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | C7D12 |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 22 kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human RAB1B between 1-201 amino acids. |
| 亞 型 | IgG1, k |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196]; Alvarez et al., 2003 [PubMed 12802079]).[supplied by OMIM, Jan 2009] Function: Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Subcellular Location: Membrane. Cytoplasm. Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments. In the GDP-form, colocalizes with GDI in the cytoplasm. Post-translational modifications: Prenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1). AMPylation at Tyr-77 occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB1B constitutively active. Similarity: Belongs to the small GTPase superfamily. Rab family. SWISS: Q9H0U4 Gene ID: 81876 Database links: Entrez Gene: 81876 Human SwissProt: Q9H0U4 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Hela cell lysates
Lane 2: A431 cell lysates
Lane 3: U-20S cell lysates
Lane 4: Rat liver tissue lysates
Lane 5: NIH/3T3 cell lysates
Primary: Anti-RAB1B (bsm-51615M) at 1/4000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 25 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
