| 產(chǎn)品編號 | bsm-55522R |
| 英文名稱 | S100A4 Recombinant Rabbit mAb |
| 中文名稱 | S100鈣結(jié)合蛋白A4重組兔單抗 |
| 別 名 | 18A2; 42A; CAPL; FSP1; MTS1; P9KA; PEL98; S100 calcium binding protein A4 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | SB100 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1: 500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 12 kDa |
| 檢測分子量 | 12 |
| 性 狀 | Liquid |
| 免 疫 原 | Recombinant Mouse S100A4 protein |
| 亞 型 | IgG |
| 純化方法 | Affinity Purification |
| 緩 沖 液 | PBS with 100 μg/ml BSA,0.15% ProClin300 and 50% glycerol. |
| 保存條件 | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. SWISS: P07091 Gene ID: 20198 S100A4屬于鈣離子結(jié)合蛋白S100蛋白家族，參與細胞骨架及細胞膜的相互作用、鈣離子信號傳遞和細胞生長及分化等，在細胞內(nèi)、外均可發(fā)揮作用，增加腫瘤細胞的運動、侵襲能力，降低細胞間黏附力，重塑細胞外基質(zhì)，促進細胞異常增生及血管生成，被稱為轉(zhuǎn)移促進基因。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with S100A4 Monoclonal Antibody, Unconjugated (bsm-55522R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with S100A4 Monoclonal Antibody, Unconjugated (bsm-55522R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with S100A4 Monoclonal Antibody, Unconjugated (bsm-55522R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with S100A4 Monoclonal Antibody, Unconjugated (bsm-55522R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with S100A4 Monoclonal Antibody, Unconjugated (bsm-55522R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with S100A4 Monoclonal Antibody, Unconjugated (bsm-55522R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with S100A4 Monoclonal Antibody, Unconjugated (bsm-55522R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with S100A4 Monoclonal Antibody, Unconjugated (bsm-55522R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with S100A4 Monoclonal Antibody, Unconjugated (bsm-55522R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
